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TYRO3 ELISA Kit

TYRO3 Reaktivität: Human Colorimetric Sandwich ELISA 40-10,000 pg/mL Cell Culture Supernatant, Serum
Produktnummer ABIN625289
  • Target Alle TYRO3 ELISA Kits anzeigen
    TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
    Reaktivität
    • 4
    • 4
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    40-10,000 pg/mL
    Untere Nachweisgrenze
    40 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Produktmerkmale
    Custom Human Dtk ELISA Kit available on demand
    Bestandteile
    Pre-Coated 96-Well Strip Microplate
    Wash Buffer
    Stop Solution
    Assay Diluent(s)
    Lyophilized Standard
    Biotinylated Detection Antibody
    Streptavidin-Conjugated HRP
    TMB One-Step Substrate
    Benötigtes Material
    Distilled or deionized water
    100 mL and 1 liter graduated cylinders
    Tubes to prepare sample dilutions
    Protease and Phosphatase inhibitors
    Precision pipettes to deliver 2 μL to 1 mL volumes
    Adjustable 1-25 mL pipettes for reagent preparation
    Benchtop rocker or shaker
    Microplate reader capable of measuring absorbance at 450 nm
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    Prepare all reagents, samples and standards as instructed in the manual.
    Add 100 µl of standard or sample to each well.
    Incubate 2.5 h at RT or O/N at 4°C.
    Add 100 µl of prepared biotin antibody to each well.
    Incubate 1 h at RT.
    Add 100 µl of prepared Streptavidin solution to each well.
    Incubate 45 min at RT.
    Add 100 µl of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 µl of Stop Solution to each well.
    Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target Alle TYRO3 ELISA Kits anzeigen
    TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
    Andere Bezeichnung
    Dtk (TYRO3 Produkte)
    Synonyme
    TYRO3 ELISA Kit, brt ELISA Kit, byk ELISA Kit, dtk ELISA Kit, rse ELISA Kit, sky ELISA Kit, tif ELISA Kit, Xksy ELISA Kit, MGC145734 ELISA Kit, DKFZp469I0432 ELISA Kit, LOC100231598 ELISA Kit, BYK ELISA Kit, Dtk ELISA Kit, RSE ELISA Kit, Sky ELISA Kit, Tif ELISA Kit, Brt ELISA Kit, REK ELISA Kit, ksy ELISA Kit, xksy ELISA Kit, AI323366 ELISA Kit, Etk-2 ELISA Kit, Rse ELISA Kit, TYRO3 protein tyrosine kinase ELISA Kit, TYRO3 protein tyrosine kinase L homeolog ELISA Kit, TYRO3 protein tyrosine kinase 3 ELISA Kit, TYRO3 ELISA Kit, tyro3 ELISA Kit, Tyro3 ELISA Kit, tyro3.L ELISA Kit
    Pathways
    RTK Signalweg
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