H2AFX ELISA Kit

Produktnummer ABIN6228783

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human H2AFX.

Kurzübersicht für H2AFX ELISA Kit (ABIN6228783)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails H2AFX ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Human Histone H2AX (H2AFX) ELISA Kit

Analytische Methode: Quantitative

Sensitivität: 47 pg/mL

Bestandteile: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Benötigtes Material:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Anwendungsinformationen

Applikationshinweise: Optimal dilutions/concentrations should be determined by the end user.

Kommentare:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plattentyp: Pre-coated

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Histone H2AX (H2AFX) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Histone H2AX (H2AFX) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Haltbarkeit: 6 months

Details zu H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Andere Bezeichnung: Histone H2AX

Hintergrund: H2AX_HUMAN

Gen-ID: 3014

HGNC: 4739

OMIM: 601772

UniProt: P16104

Pathways: Telomere Maintenance, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus