H+K+-ATPase Activity Assay Kit

Produktnummer ABIN7121129

Kurzübersicht für H+K+-ATPase Activity Assay Kit (ABIN7121129)

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Nachweismethode: Colorimetric

Applikation: Activity Assay (AcA)

Proben: Cell Samples, Tissue Samples

Analytische Methode: Quantitative

Produktdetails

Verwendungszweck: The H+/K+-ATPase Assay Kit is biochemical kit in Colorimetric method

Produktmerkmale: ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

Benötigtes Material: Spectrophotometry (Visible range)

Anwendungsinformationen

Testdauer: 1 h

Testpräzision:

Inter-assay precision: 9.8%

Intra-assay precision: 4.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect from light.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Haltbarkeit: 12 months

Antigendetails

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Andere Bezeichnung: H+K+-ATPase