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ATP4b ELISA Kit

ATP4b Reaktivität: Ratte Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Produktnummer ABIN5674849
  • Target Alle ATP4b ELISA Kits anzeigen
    ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))
    Reaktivität
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0136 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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    Discover our top product ATP4b ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle ATP4b ELISA Kits anzeigen
    ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))
    Andere Bezeichnung
    ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) (ATP4b Produkte)
    Synonyme
    AV080843 ELISA Kit, S76401S1 ELISA Kit, ATP6B ELISA Kit, ATPase, H+/K+ exchanging, beta polypeptide ELISA Kit, ATPase H+/K+ transporting beta subunit ELISA Kit, ATPase, H+/K+ exchanging, beta polypeptide S homeolog ELISA Kit, ATPase H+/K+ transporting alpha subunit ELISA Kit, Atp4b ELISA Kit, atp4b.S ELISA Kit, ATP4B ELISA Kit, ATP4A ELISA Kit
    Hintergrund
    Alternative name: Proton pump beta chain, Gastric H(+)/K(+) ATPase subunit beta
    Gen-ID
    24217
    UniProt
    P18598
    Pathways
    Proton Transport
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