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JUNB ELISA Kit (Jun B Proto-Oncogene) ELISA Kit

JUNB Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690902
$728.20
Zzgl. Versandkosten $45.00
96 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 31 bis 41 Werktagen
  • Target
    JUNB
    Reaktivität
    Human
    • 3
    • 2
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human JunB ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human JunB.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target
    JUNB
    Andere Bezeichnung
    JunB (JUNB ELISA Kit Abstract)
    Synonyme
    AP-1, junb, wu:cegs2778, wu:fa92h07, wu:fq24h03, zgc:64066, MGC81322, JUNB, junbl, wu:fa95h09, wu:fj44e11, wu:fj68b07, zgc:63933, JunB proto-oncogene, AP-1 transcription factor subunit, JunB proto-oncogene, AP-1 transcription factor subunit a, jun B proto-oncogene L homeolog, jun B proto-oncogene, jun B proto-oncogene S homeolog, JunB proto-oncogene, AP-1 transcription factor subunit b, JUNB, Junb, junba, junb.L, junb, junb.S, junbb
    Gen-ID
    3726
    UniProt
    P17275
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