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JUNB ELISA Kit (Jun B Proto-Oncogene) ELISA Kit

JUNB Reaktivität: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Produktnummer ABIN5526728
$480.70
Zzgl. Versandkosten $45.00
96 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 2 bis 3 Werktagen
  • Target
    JUNB
    Reaktivität
    Human
    • 3
    • 2
    Nachweismethode
    Colorimetric
    Methodentyp
    DNA-Binding ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human jun-B Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical jun-B binding sequences to semi-quantitatively detect active jun-B in lysates or nuclear extracts. Only available in North America.
    Proben
    Cell Lysate, Nuclear Extract
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The olionucleotide/antibody pair provided in this kit recognizes human jun-B in whole lysates and nuclear extracts.
    Produktmerkmale
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Bestandteile
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target
    JUNB
    Andere Bezeichnung
    JUNB (JUNB ELISA Kit Abstract)
    Synonyme
    AP-1, junb, wu:cegs2778, wu:fa92h07, wu:fq24h03, zgc:64066, MGC81322, JUNB, junbl, wu:fa95h09, wu:fj44e11, wu:fj68b07, zgc:63933, JunB proto-oncogene, AP-1 transcription factor subunit, JunB proto-oncogene, AP-1 transcription factor subunit a, jun B proto-oncogene L homeolog, jun B proto-oncogene, jun B proto-oncogene S homeolog, JunB proto-oncogene, AP-1 transcription factor subunit b, JUNB, Junb, junba, junb.L, junb, junb.S, junbb
    Gen-ID
    3726
    UniProt
    P17275
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