SRSF4 ELISA Kit

Produktnummer ABIN5674940

Produktdetails SRSF4 ELISA Kit

Target: SRSF4 (serine/arginine-Rich Splicing Factor 4 (SRSF4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0058 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SRSF4

Target: SRSF4 (serine/arginine-Rich Splicing Factor 4 (SRSF4))

Andere Bezeichnung: Serine/Arginine Rich Splicing Factor 4 (SRSF4) (SRSF4 Produkte)

Synonyme: SFRS4 ELISA Kit, SRP75 ELISA Kit, 5730499P16Rik ELISA Kit, AW550192 ELISA Kit, MNCb-2616 ELISA Kit, SRp75 ELISA Kit, Sfrs4 ELISA Kit, RGD1561347 ELISA Kit, serine and arginine rich splicing factor 4 ELISA Kit, serine/arginine-rich splicing factor 4 ELISA Kit, SRSF4 ELISA Kit, Srsf4 ELISA Kit

Hintergrund: Alternative name: SFRS4, SRP75, SRP001LB, Pre-mRNA-splicing factor SRP75, Splicing factor, arginine/serine-rich 4

Gen-ID: 6429

UniProt: Q08170