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MASP2 ELISA Kit

MASP2 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Produktnummer ABIN5674934
  • Target Alle MASP2 ELISA Kits anzeigen
    MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
    Reaktivität
    • 4
    • 2
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Sensitivität
    0067 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle MASP2 ELISA Kits anzeigen
    MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
    Andere Bezeichnung
    Mannose Associated Serine Protease 2 (MASP2) (MASP2 Produkte)
    Synonyme
    MAP19 ELISA Kit, MASP-2 ELISA Kit, MASP1P1 ELISA Kit, sMAP ELISA Kit, MAp19 ELISA Kit, mannan binding lectin serine peptidase 2 ELISA Kit, mannan-binding lectin serine peptidase 2 ELISA Kit, MASP2 ELISA Kit, Masp2 ELISA Kit
    Hintergrund
    Alternative name: Mannose-binding protein-associated serine protease 2, mannan-Binding Lectin Serine Peptidase 2, MBL-associated serine protease 2
    Gen-ID
    17175
    UniProt
    Q91WP0
    Pathways
    Komplementsystem
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