ARPC4 ELISA Kit

Produktnummer ABIN5674866

Produktdetails ARPC4 ELISA Kit

Target: ARPC4 (Actin Related Protein 2/3 Complex, Subunit 4, 20kDa (ARPC4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0115 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ARPC4

Target: ARPC4 (Actin Related Protein 2/3 Complex, Subunit 4, 20kDa (ARPC4))

Andere Bezeichnung: Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) (ARPC4 Produkte)

Synonyme: ARC20 ELISA Kit, P20-ARC ELISA Kit, 20kDa ELISA Kit, 5330419I20Rik ELISA Kit, AI327076 ELISA Kit, p20-Arc ELISA Kit, arc20 ELISA Kit, p20-arc ELISA Kit, ARPC4 ELISA Kit, fa07b12 ELISA Kit, wu:fa07b12 ELISA Kit, zgc:77932 ELISA Kit, actin related protein 2/3 complex subunit 4 ELISA Kit, Actin-related protein 2/3 complex subunit 4 ELISA Kit, actin-related protein 2/3 complex subunit 4 ELISA Kit, actin related protein 2/3 complex, subunit 4 ELISA Kit, actin related protein 2/3 complex subunit 4 S homeolog ELISA Kit, actin related protein 2/3 complex, subunit 4, 20kDa ELISA Kit, ARPC4 ELISA Kit, PTRG_00644 ELISA Kit, CMU_000110 ELISA Kit, Arpc4 ELISA Kit, arpc4 ELISA Kit, arpc4.S ELISA Kit

Hintergrund: Alternative name: ARC20, p20-Arc, Arp2/3 Protein Complex Subunit p20, Arp2/3 complex 20 kDa subunit

Gen-ID: 100526693

UniProt: P59998

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization