PRKDC ELISA Kit

Produktnummer ABIN5674824

Produktdetails PRKDC ELISA Kit

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0125 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Andere Bezeichnung: Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) (PRKDC Produkte)

Synonyme: DNA-PKcs ELISA Kit, DNAPK ELISA Kit, DNPK1 ELISA Kit, HYRC ELISA Kit, HYRC1 ELISA Kit, XRCC7 ELISA Kit, p350 ELISA Kit, DNA-PKCS ELISA Kit, dna-pkcs ELISA Kit, dnapk ELISA Kit, dnpk1 ELISA Kit, hyrc ELISA Kit, hyrc1 ELISA Kit, prkdc-A ELISA Kit, xrcc7 ELISA Kit, PRKDC ELISA Kit, AI326420 ELISA Kit, AU019811 ELISA Kit, DNAPDcs ELISA Kit, DOXNPH ELISA Kit, dxnph ELISA Kit, p460 ELISA Kit, scid ELISA Kit, slip ELISA Kit, protein kinase, DNA-activated, catalytic polypeptide ELISA Kit, protein kinase, DNA-activated, catalytic polypeptide L homeolog ELISA Kit, DNA-dependent protein kinase catalytic subunit ELISA Kit, protein kinase, DNA activated, catalytic polypeptide ELISA Kit, PRKDC ELISA Kit, prkdc.L ELISA Kit, LOC100633457 ELISA Kit, Prkdc ELISA Kit

Hintergrund: Alternative name: HYRC, HYRC1, DNPK1, p350, DNAPK, XRCC7, DNA-PKcs

Gen-ID: 5591

UniProt: P78527

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response