SOCS2 ELISA Kit

Produktnummer ABIN5674724

Produktdetails SOCS2 ELISA Kit

Target: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0066 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SOCS2

Target: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Andere Bezeichnung: Suppressors Of Cytokine Signaling 2 (SOCS2) (SOCS2 Produkte)

Synonyme: CIS2 ELISA Kit, Cish2 ELISA Kit, SOCS-2 ELISA Kit, SSI-2 ELISA Kit, SSI2 ELISA Kit, STATI2 ELISA Kit, Cish1 ELISA Kit, Socs-1 ELISA Kit, Socs-2 ELISA Kit, si:dkey-202b22.1 ELISA Kit, socs2 ELISA Kit, cis2 ELISA Kit, ssi2 ELISA Kit, cish2 ELISA Kit, ssi-2 ELISA Kit, socs-2 ELISA Kit, stati2 ELISA Kit, 8030460M17 ELISA Kit, AI527257 ELISA Kit, AW108012 ELISA Kit, D130043N08Rik ELISA Kit, JAB ELISA Kit, hg ELISA Kit, Cradd ELISA Kit, Socs2 ELISA Kit, suppressor of cytokine signaling 2 ELISA Kit, suppressor of cytokine signaling 1 ELISA Kit, death domain-containing protein CRADD ELISA Kit, suppressor of cytokine signaling 2 S homeolog ELISA Kit, SOCS2 ELISA Kit, Socs1 ELISA Kit, Socs2 ELISA Kit, socs2 ELISA Kit, LOC100716104 ELISA Kit, socs2.S ELISA Kit

Hintergrund: Alternative name: CIS2, Cish2, SOCS-2, SSI-2, SSI2, STATI2, Cytokine-inducible SH2 protein 2, STAT-induced STAT inhibitor 2

Gen-ID: 216233

UniProt: O35717

Pathways: JAK-STAT Signalweg, Response to Growth Hormone Stimulus