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MAT1A ELISA Kit

MAT1A Reaktivität: Human Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Tissue Homogenate
Produktnummer ABIN5674721
  • Target Alle MAT1A ELISA Kits anzeigen
    MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))
    Reaktivität
    • 3
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.625 ng/mL - 40 ng/mL
    Untere Nachweisgrenze
    0.625 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0255 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle MAT1A ELISA Kits anzeigen
    MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))
    Andere Bezeichnung
    Methionine Adenosyltransferase I Alpha (MAT1a) (MAT1A Produkte)
    Synonyme
    MAT ELISA Kit, MATA1 ELISA Kit, SAMS ELISA Kit, SAMS1 ELISA Kit, AdoMet ELISA Kit, SADE ELISA Kit, SAS ELISA Kit, AI046368 ELISA Kit, Ams ELISA Kit, wu:fi35e01 ELISA Kit, zgc:55442 ELISA Kit, methionine adenosyltransferase 1A ELISA Kit, methionine adenosyltransferase I, alpha ELISA Kit, MAT1A ELISA Kit, Mat1a ELISA Kit, mat1a ELISA Kit
    Hintergrund
    Alternative name: MAT, SAMS, MATA1, SAMS1, AMS1, S-Adenosylmethionine Synthetase, S-adenosylmethionine synthase isoform type-1
    Gen-ID
    4143
    UniProt
    Q00266
    Pathways
    Mitotic G1-G1/S Phases, M Phase, Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process
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