OPTN ELISA Kit

Produktnummer ABIN5674718

Produktdetails OPTN ELISA Kit

Target: OPTN (Optineurin (OPTN))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.125 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 32.4 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu OPTN

Target: OPTN (Optineurin (OPTN))

Abstract: OPTN Produkte

Synonyme: nrp ELISA Kit, fip2 ELISA Kit, hip7 ELISA Kit, hypl ELISA Kit, glc1e ELISA Kit, tfiiia-intp ELISA Kit, ALS12 ELISA Kit, FIP2 ELISA Kit, GLC1E ELISA Kit, HIP7 ELISA Kit, HYPL ELISA Kit, NRP ELISA Kit, TFIIIA-INTP ELISA Kit, Fip2 ELISA Kit, 4930441O07Rik ELISA Kit, fj52f04 ELISA Kit, si:ch211-240l19.3 ELISA Kit, wu:fj52f04 ELISA Kit, zgc:66386 ELISA Kit, zgc:77868 ELISA Kit, optineurin ELISA Kit, optineurin L homeolog ELISA Kit, OPTN ELISA Kit, optn ELISA Kit, Optn ELISA Kit, optn.L ELISA Kit

Hintergrund: Alternative name: NRP, FIP2, GLC1E, HIP7, HYPL, TFIIIA-INTP, Glaucoma 1,Open Angle,E(Adult-Onset), Huntingtin-interacting protein 7, Optic neuropathy-inducing protein, E3-14.7K-interacting protein

Gen-ID: 246294

UniProt: Q8R5M4

Pathways: M Phase