LYN ELISA Kit

Produktnummer ABIN5659821

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human LYN.

Kurzübersicht für LYN ELISA Kit (ABIN5659821)

Target: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Produktdetails LYN ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN). No significant cross-reactivity or interference between V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) and analogues was observed.

Sensitivität: 0.062 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu LYN

Target: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Andere Bezeichnung: V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog

Hintergrund:

Gene Name: V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog

Gene Aliases: JTK8, p53Lyn, p56Lyn, Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase

Gen-ID: 4067

UniProt: P07948

Pathways: Fc-epsilon Rezeptor Signalübertragung, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex, BCR Signaling