URGCP ELISA Kit

Produktnummer ABIN5659761

Produktdetails URGCP ELISA Kit

Target: URGCP (Upregulator of Cell Proliferation (URGCP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Upregulator Of Cell Proliferation (URGCP). No significant cross-reactivity or interference between Upregulator Of Cell Proliferation (URGCP) and analogues was observed.

Sensitivität: 0.061 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Upregulator Of Cell Proliferation (URGCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Upregulator Of Cell Proliferation (URGCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Upregulator Of Cell Proliferation (URGCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Upregulator Of Cell Proliferation (URGCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Upregulator Of Cell Proliferation (URGCP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Upregulator Of Cell Proliferation (URGCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu URGCP

Target: URGCP (Upregulator of Cell Proliferation (URGCP))

Andere Bezeichnung: Upregulator Of Cell Proliferation (URGCP Produkte)

Synonyme: URG4 ELISA Kit, DKFZp469D1721 ELISA Kit, urg4 ELISA Kit, MGC132266 ELISA Kit, 2010005J08Rik ELISA Kit, 9130001I21Rik ELISA Kit, AW060359 ELISA Kit, Urg4 ELISA Kit, mKIAA1507 ELISA Kit, RGD1564681 ELISA Kit, up-regulator of cell proliferation ELISA Kit, upregulator of cell proliferation ELISA Kit, LOC610578 ELISA Kit, LOC100064842 ELISA Kit, URGCP ELISA Kit, urgcp ELISA Kit, Urgcp ELISA Kit

Hintergrund:

Gene Name: Upregulator Of Cell Proliferation

Gene Aliases: URG4, Up Regulated Gene 4, HBV X protein up-regulated gene 4 protein, HBxAg up-regulated gene 4 protein

Gen-ID: 72046

UniProt: Q5NCI0