URGCP ELISA Kit

Produktnummer ABIN2044755

Produktdetails URGCP ELISA Kit

Target: URGCP (Upregulator of Cell Proliferation (URGCP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.061 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Upregulator Of Cell Proliferation (URGCP) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Upregulator Of Cell Proliferation (URGCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Upregulator Of Cell Proliferation (URGCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Upregulator Of Cell Proliferation (URGCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Upregulator Of Cell Proliferation (URGCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu URGCP

Target: URGCP (Upregulator of Cell Proliferation (URGCP))

Abstract: URGCP Produkte

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