Thrombospondin 1 CLIA Kit

Produktnummer ABIN5659181

Produktdetails Thrombospondin 1 CLIA Kit

Target: Thrombospondin 1 (THBS1)

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Plasma

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Thrombospondin 1 (THBS1). No significant cross-reactivity or interference between Thrombospondin 1 (THBS1) and analogues was observed.

Sensitivität: 2.28 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 1 (THBS1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thrombospondin 1 (THBS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thrombospondin 1 (THBS1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombospondin 1 (THBS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombospondin 1 (THBS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Thrombospondin 1 (THBS1)

Andere Bezeichnung: Thrombospondin 1 (THBS1 Produkte)

Synonyme: THBS CLIA Kit, THBS-1 CLIA Kit, TSP CLIA Kit, TSP-1 CLIA Kit, TSP1 CLIA Kit, Thbs-1 CLIA Kit, tbsp1 CLIA Kit, thrombospondin-1 CLIA Kit, thbs CLIA Kit, thbs-1 CLIA Kit, tsp CLIA Kit, tsp-1 CLIA Kit, tsp1 CLIA Kit, Tsp1 CLIA Kit, THBS1 CLIA Kit, Tsp-1 CLIA Kit, wu:fc48c03 CLIA Kit, si:dkey-11e23.1 CLIA Kit, tsp2 CLIA Kit, thrombospondin 1 CLIA Kit, thrombospondin 1 S homeolog CLIA Kit, thrombospondin 1b CLIA Kit, thrombospondin 2 L homeolog CLIA Kit, THBS1 CLIA Kit, Thbs1 CLIA Kit, thbs1.S CLIA Kit, thbs1b CLIA Kit, thbs1 CLIA Kit, thbs2.L CLIA Kit

Hintergrund:

Gene Name: Thrombospondin 1

Gene Aliases: TSP1, Thrombospondin-1p180

UniProt: P35441

Pathways: Autophagie