KIT CLIA Kit

Produktnummer ABIN5658954

Produktdetails KIT CLIA Kit

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 468.75 pg/mL - 30000 pg/mL

Untere Nachweisgrenze: 468.75 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor Receptor (SCFR). No significant cross-reactivity or interference between Stem Cell Factor Receptor (SCFR) and analogues was observed.

Sensitivität: 15.3 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor Receptor (SCFR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stem Cell Factor Receptor (SCFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stem Cell Factor Receptor (SCFR) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: Stem Cell Factor Receptor (KIT Produkte)

Synonyme: C-Kit CLIA Kit, CD117 CLIA Kit, PBT CLIA Kit, SCFR CLIA Kit, Bs CLIA Kit, Fdc CLIA Kit, Gsfsco1 CLIA Kit, Gsfsco5 CLIA Kit, Gsfsow3 CLIA Kit, SCO1 CLIA Kit, SCO5 CLIA Kit, SOW3 CLIA Kit, Ssm CLIA Kit, Tr-kit CLIA Kit, W CLIA Kit, c-KIT CLIA Kit, c-kit CLIA Kit, MGF CLIA Kit, KIT CLIA Kit, C-KIT CLIA Kit, kit CLIA Kit, receptor CLIA Kit, sparse CLIA Kit, Xkl-1 CLIA Kit, kit-a CLIA Kit, kit-b CLIA Kit, kl1-A CLIA Kit, krk1 CLIA Kit, pbt CLIA Kit, scfr CLIA Kit, KIT proto-oncogene receptor tyrosine kinase CLIA Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a CLIA Kit, KIT proto-oncogene receptor tyrosine kinase S homeolog CLIA Kit, KIT CLIA Kit, Kit CLIA Kit, kita CLIA Kit, kit CLIA Kit, kit.S CLIA Kit

Hintergrund:

Gene Name: Stem Cell Factor Receptor

Gene Aliases: CD117, KIT, C-Kit, PBT, p145 c-Kit, C-Kit Receptor, Tyrosine-Protein Kinase, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, Piebald Trait

Gen-ID: 3815

UniProt: P10721

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity