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LKB1 ELISA Kit

STK11 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5658730
  • Target Alle LKB1 (STK11) ELISA Kits anzeigen
    LKB1 (STK11) (serine/threonine Kinase 11 (STK11))
    Reaktivität
    • 8
    • 3
    • 2
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Serine/Threonine Kinase 11 (STK11). No significant cross-reactivity or interference between Serine/Threonine Kinase 11 (STK11) and analogues was observed.
    Sensitivität
    0.051 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 11 (STK11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 11 (STK11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Threonine Kinase 11 (STK11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Threonine Kinase 11 (STK11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 11 (STK11) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 11 (STK11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle LKB1 (STK11) ELISA Kits anzeigen
    LKB1 (STK11) (serine/threonine Kinase 11 (STK11))
    Andere Bezeichnung
    Serine/Threonine Kinase 11 (STK11 Produkte)
    Synonyme
    pjs ELISA Kit, LKB1 ELISA Kit, XEEK1 ELISA Kit, Stk11 ELISA Kit, PJS ELISA Kit, hLKB1 ELISA Kit, AA408040 ELISA Kit, Lkb1 ELISA Kit, Par-4 ELISA Kit, R75140 ELISA Kit, mLKB1 ELISA Kit, wu:fj61a05 ELISA Kit, zgc:110180 ELISA Kit, xeek1 ELISA Kit, serine/threonine kinase 11 ELISA Kit, polarization-related protein LKB1 ELISA Kit, serine/threonine kinase 11 L homeolog ELISA Kit, STK11 ELISA Kit, stk11 ELISA Kit, LOC662493 ELISA Kit, Stk11 ELISA Kit, stk11.L ELISA Kit
    Hintergrund

    Gene Name: Serine/Threonine Kinase 11

    Gene Aliases: LKB1, PJS, Polarization-Related Protein LKB1, Peutz-Jeghers Syndrome, Liver kinase B1, Renal carcinoma antigen NY-REN-19

    Gen-ID
    20869
    UniProt
    Q9WTK7
    Pathways
    AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt
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