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SFRP2 ELISA Kit

SFRP2 Reaktivität: Human Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5658626
  • Target Alle SFRP2 ELISA Kits anzeigen
    SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
    Reaktivität
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.25 ng/mL - 80 ng/mL
    Untere Nachweisgrenze
    1.25 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 2 (SFRP2). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 2 (SFRP2) and analogues was observed.
    Sensitivität
    0.53 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 2 (SFRP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 2 (SFRP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 2 (SFRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 2 (SFRP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 2 (SFRP2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 2 (SFRP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle SFRP2 ELISA Kits anzeigen
    SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
    Andere Bezeichnung
    Secreted Frizzled Related Protein 2 (SFRP2 Produkte)
    Synonyme
    frp-2 ELISA Kit, sarp1 ELISA Kit, sdf-5 ELISA Kit, sfrp-2 ELISA Kit, SFRP2 ELISA Kit, sfrp2 ELISA Kit, FRP-2 ELISA Kit, SARP1 ELISA Kit, SDF-5 ELISA Kit, AI851596 ELISA Kit, Sdf5 ELISA Kit, hm:zeh0225 ELISA Kit, zeh0225 ELISA Kit, zgc:153618 ELISA Kit, SFRP-2 ELISA Kit, secreted frizzled-related protein 2 ELISA Kit, secreted frizzled related protein 2 ELISA Kit, secreted frizzled-related protein 2 S homeolog ELISA Kit, secreted frizzled-related protein ELISA Kit, sfrp2 ELISA Kit, sfrp2 ELISA Kit, SFRP2 ELISA Kit, sfrp2.S ELISA Kit, LOAG_01461 ELISA Kit, Sfrp2 ELISA Kit
    Hintergrund

    Gene Name: Secreted Frizzled Related Protein 2

    Gene Aliases: FRP-2, SARP1, SDF-5, Secreted apoptosis-related protein 1

    Pathways
    WNT Signalweg, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation
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