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RHOA ELISA Kit

RHOA Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5658356
  • Target Alle RHOA ELISA Kits anzeigen
    RHOA (Ras Homolog Gene Family, Member A (RHOA))
    Reaktivität
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Ras Homolog Gene Family, Member A (RHOA). No significant cross-reactivity or interference between Ras Homolog Gene Family, Member A (RHOA) and analogues was observed.
    Sensitivität
    0.065 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Homolog Gene Family, Member A (RHOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Homolog Gene Family, Member A (RHOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Homolog Gene Family, Member A (RHOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ras Homolog Gene Family, Member A (RHOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Homolog Gene Family, Member A (RHOA) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Homolog Gene Family, Member A (RHOA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle RHOA ELISA Kits anzeigen
    RHOA (Ras Homolog Gene Family, Member A (RHOA))
    Andere Bezeichnung
    Ras Homolog Gene Family, Member A (RHOA Produkte)
    Synonyme
    ARH12 ELISA Kit, ARHA ELISA Kit, RHO12 ELISA Kit, RHOH12 ELISA Kit, Arha ELISA Kit, Arha1 ELISA Kit, Arha2 ELISA Kit, arha ELISA Kit, arha2 ELISA Kit, xrhoa ELISA Kit, arh12 ELISA Kit, rho12 ELISA Kit, rhoh12 ELISA Kit, rhoa ELISA Kit, RHO1 ELISA Kit, Rhoa ELISA Kit, wu:fa66g04 ELISA Kit, wu:fb26g07 ELISA Kit, zgc:66058 ELISA Kit, zgc:85805 ELISA Kit, ARHC ELISA Kit, rhoae ELISA Kit, zgc:64015 ELISA Kit, ras homolog family member A ELISA Kit, ras homolog family member A S homeolog ELISA Kit, ras homolog family member A L homeolog ELISA Kit, ras homolog gene family, member Aa ELISA Kit, ras homolog family member Ca ELISA Kit, RHOA ELISA Kit, Rhoa ELISA Kit, rhoa.S ELISA Kit, rhoa ELISA Kit, rhoa.L ELISA Kit, rhoaa ELISA Kit, rhoca ELISA Kit
    Hintergrund

    Gene Name: Ras Homolog Gene Family, Member A

    Gene Aliases: ARH12, ARHA, RHO12, RHOH12, Rho cDNA clone 12

    Pathways
    Microtubule Dynamics, WNT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interaktom
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