PKC epsilon CLIA Kit

Produktnummer ABIN5658222

Produktdetails PKC epsilon CLIA Kit

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Kinase C Epsilon (PKCe). No significant cross-reactivity or interference between Protein Kinase C Epsilon (PKCe) and analogues was observed.

Sensitivität: 0.07 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein Kinase C Epsilon (PKCe) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Andere Bezeichnung: Protein Kinase C Epsilon (PRKCE Produkte)

Synonyme: PKCE CLIA Kit, nPKC-epsilon CLIA Kit, 5830406C15Rik CLIA Kit, PKC[e] CLIA Kit, PKCepsilon CLIA Kit, Pkce CLIA Kit, R75156 CLIA Kit, fj59b07 CLIA Kit, prkce CLIA Kit, wu:fj59b07 CLIA Kit, pkce CLIA Kit, pkc-epsilon CLIA Kit, PRKCH CLIA Kit, protein kinase C epsilon CLIA Kit, protein kinase C, epsilon CLIA Kit, protein kinase C, epsilon a CLIA Kit, PRKCE CLIA Kit, Prkce CLIA Kit, prkcea CLIA Kit, prkce CLIA Kit

Hintergrund:

Gene Name: Protein Kinase C Epsilon

Gene Aliases: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon

Gen-ID: 5581

UniProt: Q02156

Pathways: T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling