P4HB CLIA Kit

Produktnummer ABIN5658198

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Human P4HB.

Kurzübersicht für P4HB CLIA Kit (ABIN5658198)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 13.7 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Produktdetails P4HB CLIA Kit

Untere Nachweisgrenze: 13.7 pg/mL

Verwendungszweck: Human Protein Disulfide Isomerase (PDI) CLIA Kit for detecting PDI in tissue homogenates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A2 (PDIA2). No significant cross-reactivity or interference between Protein Disulfide Isomerase A2 (PDIA2) and analogues was observed.

Sensitivität: 5.9 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A2 (PDIA2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A2 (PDIA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein Disulfide Isomerase A2 (PDIA2) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A2 (PDIA2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A2 (PDIA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: Protein Disulfide Isomerase

Hintergrund: Gene Aliases: PDI-A2, PDA2, PDIA2, PDIR, PDIP, Protein Disulfide Isomerase,Pancreatic, Protein Disulfide Isomerase-Associated 2, Pancreas-specific protein disulfide isomerase

Gen-ID: 64714

UniProt: Q13087

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism