PAI1 CLIA Kit

Produktnummer ABIN5657860

Produktdetails PAI1 CLIA Kit

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 312.5 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312.5 pg/mL

Applikation: ELISA

Proben: Plasma

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator Inhibitor 1 (PAI1). No significant cross-reactivity or interference between Plasminogen Activator Inhibitor 1 (PAI1) and analogues was observed.

Sensitivität: 11.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Plasminogen Activator Inhibitor 1 (PAI1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Andere Bezeichnung: Plasminogen Activator Inhibitor 1 (SERPINE1 Produkte)

Synonyme: PAI CLIA Kit, PAI-1 CLIA Kit, PAI1 CLIA Kit, PLANH1 CLIA Kit, SERPINE1 CLIA Kit, si:ch211-138a11.1 CLIA Kit, Planh1 CLIA Kit, PAI1A CLIA Kit, Pai1 CLIA Kit, Pai1aa CLIA Kit, Planh CLIA Kit, RATPAI1A CLIA Kit, serpin family E member 1 CLIA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog CLIA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 CLIA Kit, serine (or cysteine) peptidase inhibitor, clade E, member 1 CLIA Kit, SERPINE1 CLIA Kit, serpine1.L CLIA Kit, serpine1 CLIA Kit, Serpine1 CLIA Kit

Hintergrund:

Gene Name: Plasminogen Activator Inhibitor 1

Gene Aliases: SERPINE1, PLANH1, Serpin Peptidase Inhibitor Clade E Member 1, Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1, Endothelial plasminogen activator inhibitor

Gen-ID: 24617

UniProt: P20961

Pathways: p53 Signalweg, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie, Smooth Muscle Cell Migration