PDE3A ELISA Kit

Produktnummer ABIN5657766

Produktdetails PDE3A ELISA Kit

Target: PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 3A, cGMP Inhibited (PDE3A). No significant cross-reactivity or interference between Phosphodiesterase 3A, cGMP Inhibited (PDE3A) and analogues was observed.

Sensitivität: 0.27 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 3A, cGMP Inhibited (PDE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 3A, cGMP Inhibited (PDE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 3A, cGMP Inhibited (PDE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 3A, cGMP Inhibited (PDE3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 3A, cGMP Inhibited (PDE3A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 3A, cGMP Inhibited (PDE3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PDE3A

Target: PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))

Andere Bezeichnung: Phosphodiesterase 3A, cGMP Inhibited (PDE3A Produkte)

Synonyme: PDE3A ELISA Kit, Pde3a ELISA Kit, si:dkey-48h7.2 ELISA Kit, A930022O17Rik ELISA Kit, C87899 ELISA Kit, RNPDE3A ELISA Kit, CGI-PDE ELISA Kit, CGI-PDE A ELISA Kit, CGI-PDE-A ELISA Kit, phosphodiesterase 3A ELISA Kit, phosphodiesterase 3A, cGMP-inhibited ELISA Kit, cGMP-inhibited 3',5'-cyclic phosphodiesterase A ELISA Kit, phosphodiesterase 3A, cGMP inhibited ELISA Kit, PDE3A ELISA Kit, Pde3a ELISA Kit, LOC100540515 ELISA Kit, pde3a ELISA Kit

Hintergrund:

Gene Name: Phosphodiesterase 3A, cGMP Inhibited

Gene Aliases: CGI-PDE, Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gen-ID: 50678

UniProt: Q62865

Pathways: cAMP Metabolic Process