OCT4 ELISA Kit

Produktnummer ABIN5657492

Produktdetails OCT4 ELISA Kit

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Octamer Binding Transcription Factor 4 (OCT4). No significant cross-reactivity or interference between Octamer Binding Transcription Factor 4 (OCT4) and analogues was observed.

Sensitivität: 0.061 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Octamer Binding Transcription Factor 4 (OCT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Octamer Binding Transcription Factor 4 (OCT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: Octamer Binding Transcription Factor 4 (POU5F1 Produkte)

Synonyme: OCT3 ELISA Kit, OCT4 ELISA Kit, OTF-3 ELISA Kit, OTF3 ELISA Kit, OTF4 ELISA Kit, Oct-3 ELISA Kit, Oct-4 ELISA Kit, POU5F1 ELISA Kit, gp9 ELISA Kit, gp-9 ELISA Kit, oct4 ELISA Kit, pou2 ELISA Kit, POU-2 ELISA Kit, cb197 ELISA Kit, spg/pou2 ELISA Kit, chunp6868 ELISA Kit, wu:fd18d06 ELISA Kit, NF-A3 ELISA Kit, Oct-3/4 ELISA Kit, Oct3 ELISA Kit, Oct3/4 ELISA Kit, Oct4 ELISA Kit, Otf-3 ELISA Kit, Otf-4 ELISA Kit, Otf3 ELISA Kit, Otf3-rs7 ELISA Kit, Otf3g ELISA Kit, Otf4 ELISA Kit, POU class 5 homeobox 1 ELISA Kit, POU domain, class 5, transcription factor 3 ELISA Kit, POU domain, class 5, transcription factor 1 ELISA Kit, POU5F1 ELISA Kit, pou5f3 ELISA Kit, Pou5f1 ELISA Kit

Hintergrund:

Gene Name: Octamer Binding Transcription Factor 4

Gene Aliases: POU5F1, OCT3, OTF3, OTF4, Octamer-4, POU Domain Class 5 Transcription Factor 1, POU Class 5 Homoeobox 1, Octamer-binding protein 3

Gen-ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance