OCT4 ELISA Kit

Produktnummer ABIN5526748

Produktdetails OCT4 ELISA Kit

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: DNA-Binding ELISA

Applikation: ELISA

Verwendungszweck: Human OCT4 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical OCT4 binding sequences to semi-quantitatively detect active OCT4 in lysates or nuclear extracts. Only available in North America.

Proben: Cell Lysate, Nuclear Extract

Analytische Methode: Semi-Quantitative

Spezifität: The olionucleotide/antibody pair provided in this kit recognizes human OCT4 in whole lysates and nuclear extracts.

Produktmerkmale:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Bestandteile:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: OCT4 (POU5F1 Produkte)

Synonyme: OCT3 ELISA Kit, OCT4 ELISA Kit, OTF-3 ELISA Kit, OTF3 ELISA Kit, OTF4 ELISA Kit, Oct-3 ELISA Kit, Oct-4 ELISA Kit, POU5F1 ELISA Kit, gp9 ELISA Kit, gp-9 ELISA Kit, oct4 ELISA Kit, pou2 ELISA Kit, POU-2 ELISA Kit, cb197 ELISA Kit, spg/pou2 ELISA Kit, chunp6868 ELISA Kit, wu:fd18d06 ELISA Kit, NF-A3 ELISA Kit, Oct-3/4 ELISA Kit, Oct3 ELISA Kit, Oct3/4 ELISA Kit, Oct4 ELISA Kit, Otf-3 ELISA Kit, Otf-4 ELISA Kit, Otf3 ELISA Kit, Otf3-rs7 ELISA Kit, Otf3g ELISA Kit, Otf4 ELISA Kit, POU class 5 homeobox 1 ELISA Kit, POU domain, class 5, transcription factor 3 ELISA Kit, POU domain, class 5, transcription factor 1 ELISA Kit, POU5F1 ELISA Kit, pou5f3 ELISA Kit, Pou5f1 ELISA Kit

Gen-ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance