NLRP3 CLIA Kit

Produktnummer ABIN5657419

Das Human NLRP3 ELISA Kit (ABIN5657419) ist ein Chemiluminescent ELISA Kit zur Detektion von Human NLRP3.

Kurzübersicht für NLRP3 CLIA Kit (ABIN5657419)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 68.6 pg/mL - 50000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails NLRP3 CLIA Kit

Untere Nachweisgrenze: 68.6 pg/mL

Verwendungszweck: Human NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) CLIA Kit for detecting NLRP3 in tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). No significant cross-reactivity or interference between NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) and analogues was observed.

Sensitivität: 24.8 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLR Family, Pyrin Domain Containing Protein 3

Hintergrund: Gene Aliases: AVP, AGTAVPRL, AII, AII/AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, MWS, NALP3, PYPAF1, Cryopyrin, Cold Induced Autoinflammatory Syndrome 1, Caterpiller-Like Receptor 1.1

Gen-ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome