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NOS2 ELISA Kit

NOS2 Reaktivität: Maus Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Produktnummer ABIN5657403
  • Target Alle NOS2 ELISA Kits anzeigen
    NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
    Reaktivität
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2). No significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.
    Sensitivität
    0.116 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 2, Inducible (NOS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 2, Inducible (NOS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle NOS2 ELISA Kits anzeigen
    NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
    Andere Bezeichnung
    Nitric Oxide Synthase 2, Inducible (NOS2 Produkte)
    Synonyme
    HEP-NOS ELISA Kit, INOS ELISA Kit, NOS ELISA Kit, NOS2A ELISA Kit, NOS-II ELISA Kit, Nos-2 ELISA Kit, Nos2a ELISA Kit, i-NOS ELISA Kit, iNOS ELISA Kit, iNos ELISA Kit, NOS2a ELISA Kit, NOS2 ELISA Kit, BmNOS2 ELISA Kit, Nsl ELISA Kit, nos2 ELISA Kit, nitric oxide synthase 2 ELISA Kit, nitric oxide synthase 2, inducible ELISA Kit, inducible nitric oxide synthase ELISA Kit, inducible nitric-oxide synthase ELISA Kit, nanos C2HC-type zinc finger 2 ELISA Kit, NOS2 ELISA Kit, Nos2 ELISA Kit, nos2 ELISA Kit, LOC396821 ELISA Kit, NANOS2 ELISA Kit
    Hintergrund

    Gene Name: Nitric Oxide Synthase 2, Inducible

    Gene Aliases: NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2

    Gen-ID
    18126
    UniProt
    P29477
    Pathways
    Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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