Neuregulin 1 ELISA Kit

Produktnummer ABIN5657246

Produktdetails Neuregulin 1 ELISA Kit

Target: Neuregulin 1 (NRG1)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Neuregulin 1 (NRG1). No significant cross-reactivity or interference between Neuregulin 1 (NRG1) and analogues was observed.

Sensitivität: 6.2 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuregulin 1 (NRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuregulin 1 (NRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuregulin 1 (NRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuregulin 1 (NRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuregulin 1 (NRG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuregulin 1 (NRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Neuregulin 1

Target: Neuregulin 1 (NRG1)

Andere Bezeichnung: Neuregulin 1 (NRG1 Produkte)

Synonyme: 6030402G23Rik ELISA Kit, ARIA ELISA Kit, D230005F13Rik ELISA Kit, GGF ELISA Kit, GGFII ELISA Kit, HRG ELISA Kit, HRGalpha ELISA Kit, Hgl ELISA Kit, NDF ELISA Kit, SMDF ELISA Kit, GGF2 ELISA Kit, HGL ELISA Kit, HRG1 ELISA Kit, HRGA ELISA Kit, MST131 ELISA Kit, NRG-1 ELISA Kit, heregulin ELISA Kit, nrg1-A ELISA Kit, pro-neuregulin-1 ELISA Kit, NRG1 ELISA Kit, wu:fm70e01 ELISA Kit, DKFZp459A1838 ELISA Kit, neuregulin 1 ELISA Kit, histidine rich glycoprotein ELISA Kit, neuregulin 1 L homeolog ELISA Kit, Nrg1 ELISA Kit, NRG1 ELISA Kit, HRG ELISA Kit, nrg1.L ELISA Kit, nrg1 ELISA Kit

Hintergrund:

Gene Name: Neuregulin 1

Gene Aliases: ARIA, GGF, HGL, HRG, HRGA, NDF, SMDF, Acetylcholine receptor-inducing activity, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor

Gen-ID: 211323

UniProt: Q6DR98

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation