MCL-1 ELISA Kit

Produktnummer ABIN5657043

Produktdetails MCL-1 ELISA Kit

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). No significant cross-reactivity or interference between Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) and analogues was observed.

Sensitivität: 0.127 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Andere Bezeichnung: Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1 Produkte)

Synonyme: BCL2L3 ELISA Kit, EAT ELISA Kit, MCL1-ES ELISA Kit, MCL1L ELISA Kit, MCL1S ELISA Kit, Mcl-1 ELISA Kit, TM ELISA Kit, bcl2-L-3 ELISA Kit, mcl1/EAT ELISA Kit, AW556805 ELISA Kit, mcl-1 ELISA Kit, fb72f02 ELISA Kit, wu:fb70a08 ELISA Kit, wu:fb72f02 ELISA Kit, zfMLP1 ELISA Kit, zfMcl-1a ELISA Kit, zgc:109835 ELISA Kit, zgc:109763 ELISA Kit, E3 ELISA Kit, E3-2 ELISA Kit, RNE3 ELISA Kit, RNU107 ELISA Kit, SNORA63A ELISA Kit, elF-4AII ELISA Kit, MCL1, BCL2 family apoptosis regulator ELISA Kit, myeloid cell leukemia sequence 1 ELISA Kit, MCL1, BCL2 family apoptosis regulator a ELISA Kit, MCL1, BCL2 family apoptosis regulator b ELISA Kit, small nucleolar RNA, H/ACA box 63 ELISA Kit, MCL1 ELISA Kit, Mcl1 ELISA Kit, mcl1a ELISA Kit, mcl1b ELISA Kit, SNORA63 ELISA Kit

Hintergrund:

Gene Name: Myeloid Cell Leukemia Sequence 1, Bcl2 Related

Gene Aliases: EAT, MCL1L, MCL1S, BCL2L3, TM, Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT

Gen-ID: 4170

UniProt: Q07820

Pathways: MAPK Signalweg