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MUSK ELISA Kit

MUSK Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5657020
  • Target Alle MUSK ELISA Kits anzeigen
    MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))
    Reaktivität
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). No significant cross-reactivity or interference between Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) and analogues was observed.
    Sensitivität
    0.061 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle MUSK ELISA Kits anzeigen
    MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))
    Andere Bezeichnung
    Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK Produkte)
    Synonyme
    unp ELISA Kit, fj98e12 ELISA Kit, zgc:63467 ELISA Kit, wu:fj98e12 ELISA Kit, si:dz46m7.2 ELISA Kit, si:dz57f23.3 ELISA Kit, si:busm1-57f23.3 ELISA Kit, musk-A ELISA Kit, MuSK ELISA Kit, MUSK ELISA Kit, Mdk4 ELISA Kit, Mlk ELISA Kit, Nsk1 ELISA Kit, Nsk2 ELISA Kit, Nsk3 ELISA Kit, muscle ELISA Kit, muscle associated receptor tyrosine kinase ELISA Kit, muscle, skeletal, receptor tyrosine kinase ELISA Kit, muscle associated receptor tyrosine kinase S homeolog ELISA Kit, MUSK ELISA Kit, musk ELISA Kit, musk.S ELISA Kit, Musk ELISA Kit
    Hintergrund

    Gene Name: Muscle, Skeletal, Receptor Tyrosine Kinase

    Gene Aliases: Muscle-specific tyrosine-protein kinase receptor, Muscle-specific kinase receptor

    Gen-ID
    18198
    UniProt
    Q61006
    Pathways
    RTK Signalweg, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development
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