MBL2 ELISA Kit

Produktnummer ABIN5656639

Das Human MBL2 ELISA Kit (ABIN5656639) ist ein Colorimetric ELISA Kit zur Detektion von Human MBL2.

Kurzübersicht für MBL2 ELISA Kit (ABIN5656639)

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25 ng/mL - 400 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails MBL2 ELISA Kit

Untere Nachweisgrenze: 6.25 ng/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.

Sensitivität: 2.45 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Mannose Binding Lectin (MBL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Mannose Binding Lectin (MBL) and unlabeled Mannose Binding Lectin (MBL) (Standards or samples) with the pre-coated antibody specific to Mannose Binding Lectin (MBL). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Mannose Binding Lectin (MBL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mannose Binding Lectin (MBL) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MBL2

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Andere Bezeichnung: Mannose Binding Lectin

Hintergrund:

Gene Name: Mannose Binding Lectin

Gene Aliases: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

Gen-ID: 4153

UniProt: P11226

Pathways: Komplementsystem, Positive Regulation of Immune Effector Process