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LRP6 ELISA Kit

LRP6 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5656497
  • Target Alle LRP6 ELISA Kits anzeigen
    LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
    Reaktivität
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 6 (LRP6). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 6 (LRP6) and analogues was observed.
    Sensitivität
    0.056 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 6 (LRP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 6 (LRP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 6 (LRP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 6 (LRP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 6 (LRP6) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 6 (LRP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle LRP6 ELISA Kits anzeigen
    LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
    Andere Bezeichnung
    Low Density Lipoprotein Receptor Related Protein 6 (LRP6 Produkte)
    Synonyme
    lrp6-A ELISA Kit, Xlrp6 ELISA Kit, adcad2 ELISA Kit, lrp6 ELISA Kit, LRP6 ELISA Kit, ADCAD2 ELISA Kit, C030016K15Rik ELISA Kit, Cd ELISA Kit, Gw ELISA Kit, LDL receptor related protein 6 ELISA Kit, LDL receptor related protein 6 L homeolog ELISA Kit, LDL receptor related protein 6 S homeolog ELISA Kit, low density lipoprotein receptor-related protein 6 ELISA Kit, low-density lipoprotein receptor-related protein 6 ELISA Kit, LRP6 ELISA Kit, lrp6.L ELISA Kit, lrp6.S ELISA Kit, lrp6 ELISA Kit, Lrp6 ELISA Kit, LOC590395 ELISA Kit
    Hintergrund

    Gene Name: Low Density Lipoprotein Receptor Related Protein 6

    Gen-ID
    4040
    UniProt
    O75581
    Pathways
    WNT Signalweg, Tube Formation
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