LCAT ELISA Kit
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- Target Alle LCAT ELISA Kits anzeigen
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 62.5 pg/mL - 4000 pg/mL
- Untere Nachweisgrenze
- 62.5 pg/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Lecithin Cholesterol Acyltransferase (LCAT). No significant cross-reactivity or interference between Lecithin Cholesterol Acyltransferase (LCAT) and analogues was observed.
- Sensitivität
- 28.7 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lecithin Cholesterol Acyltransferase (LCAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lecithin Cholesterol Acyltransferase (LCAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle LCAT ELISA Kits anzeigen
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- Andere Bezeichnung
- Lecithin Cholesterol Acyltransferase (LCAT Produkte)
- Synonyme
- AI046659 ELISA Kit, D8Wsu61e ELISA Kit, MGC82035 ELISA Kit, lcat ELISA Kit, MGC88964 ELISA Kit, LCAT ELISA Kit, lecithin-cholesterol acyltransferase ELISA Kit, lecithin cholesterol acyltransferase ELISA Kit, lecithin-cholesterol acyltransferase L homeolog ELISA Kit, solute carrier family 12 member 4 ELISA Kit, fragile site, aphidicolin type, common, fra(13)(q13.2) ELISA Kit, LCAT ELISA Kit, Lcat ELISA Kit, lcat.L ELISA Kit, lcat ELISA Kit, SLC12A4 ELISA Kit, FRA13A ELISA Kit
- Hintergrund
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Gene Name: Lecithin Cholesterol Acyltransferase
Gene Aliases: phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase
- UniProt
- P18424
- Pathways
- Lipid Metabolism
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