IDH1 CLIA Kit

Produktnummer ABIN5656119

Dieses Chemiluminescent ELISA-Kit wurde entwickelt für die quantitative Messung von Human IDH1.

Kurzübersicht für IDH1 CLIA Kit (ABIN5656119)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81 ng/mL - 500 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails IDH1 CLIA Kit

Untere Nachweisgrenze: 7.81 ng/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.

Sensitivität: 0.28 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Isocitrate Dehydrogenase 1, Soluble (IDH1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Andere Bezeichnung: Isocitrate Dehydrogenase 1, Soluble

Hintergrund:

Gene Name: Isocitrate Dehydrogenase 1, Soluble

Gene Aliases: PICD, IDP, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, Cytosolic NADP-isocitrate dehydrogenase

Gen-ID: 3417

UniProt: O75874

Pathways: Warburg Effekt