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IL-34 ELISA Kit

IL34 Reaktivität: Maus Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5655963
  • Target Alle IL-34 (IL34) ELISA Kits anzeigen
    IL-34 (IL34) (Interleukin 34 (IL34))
    Reaktivität
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    7.81 pg/mL - 500 pg/mL
    Untere Nachweisgrenze
    7.81 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Interleukin 34 (IL34). No significant cross-reactivity or interference between Interleukin 34 (IL34) and analogues was observed.
    Sensitivität
    3.3 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 34 (IL34). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 34 (IL34). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 34 (IL34), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 34 (IL34) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 34 (IL34) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 34 (IL34) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle IL-34 (IL34) ELISA Kits anzeigen
    IL-34 (IL34) (Interleukin 34 (IL34))
    Andere Bezeichnung
    Interleukin 34 (IL34 Produkte)
    Synonyme
    C16orf77 ELISA Kit, IL-34 ELISA Kit, 2010004A03Rik ELISA Kit, AI593503 ELISA Kit, C18H16ORF77 ELISA Kit, interleukin 34 ELISA Kit, IL34 ELISA Kit, Il34 ELISA Kit
    Hintergrund

    Gene Name: Interleukin 34

    Gene Aliases: C16orf77

    Gen-ID
    76527
    UniProt
    Q8R1R4
    Pathways
    RTK Signalweg
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