Inhibin alpha ELISA Kit
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- Target Alle Inhibin alpha (INHA) ELISA Kits anzeigen
- Inhibin alpha (INHA) (Inhibin, alpha (INHA))
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Competition ELISA
- Detektionsbereich
- 12.35 pg/mL - 1000 pg/mL
- Untere Nachweisgrenze
- 12.35 pg/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Inhibin A (INHA). No significant cross-reactivity or interference between Inhibin A (INHA) and analogues was observed.
- Sensitivität
- 5.15 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 h
- Plattentyp
- Pre-coated
- Protokoll
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inhibin A (INHA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inhibin A (INHA) and unlabeled Inhibin A (INHA) (Standards or samples) with the pre-coated antibody specific to Inhibin A (INHA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin A (INHA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inhibin A (INHA) in the sample.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibin A (INHA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibin A (INHA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle Inhibin alpha (INHA) ELISA Kits anzeigen
- Inhibin alpha (INHA) (Inhibin, alpha (INHA))
- Andere Bezeichnung
- Inhibin A (INHA Produkte)
- Synonyme
- si:dkey-91f15.2 ELISA Kit, INHA ELISA Kit, MGC108500 ELISA Kit, AW555078 ELISA Kit, inhibin, alpha ELISA Kit, inhibin alpha subunit ELISA Kit, inhibin alpha ELISA Kit, inhibin alpha L homeolog ELISA Kit, inha ELISA Kit, INHA ELISA Kit, inha.L ELISA Kit, Inha ELISA Kit
- Hintergrund
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Gene Name: Inhibin A
Gene Aliases: INH-A
- Gen-ID
- 24504
- UniProt
- P17490
- Pathways
- Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion
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