Hexokinase 1 ELISA Kit

Produktnummer ABIN5655112

Produktdetails Hexokinase 1 ELISA Kit

Target: Hexokinase 1 (HK1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Hexokinase 1 (HK1). No significant cross-reactivity or interference between Hexokinase 1 (HK1) and analogues was observed.

Sensitivität: 27.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexokinase 1 (HK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexokinase 1 (HK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexokinase 1 (HK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hexokinase 1 (HK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexokinase 1 (HK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexokinase 1 (HK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Hexokinase 1

Target: Hexokinase 1 (HK1)

Andere Bezeichnung: Hexokinase 1 (HK1 Produkte)

Synonyme: HK1-ta ELISA Kit, HK1-tb ELISA Kit, HK1-tc ELISA Kit, HKD ELISA Kit, HKI ELISA Kit, HMSNR ELISA Kit, HXK1 ELISA Kit, Hexokinase ELISA Kit, hk1 ELISA Kit, HEX ELISA Kit, HEX-1 ELISA Kit, HK ELISA Kit, ARABIDOPSIS THALIANA HEXOKINASE 1 ELISA Kit, ATHXK1 ELISA Kit, F19B15.160 ELISA Kit, F19B15_160 ELISA Kit, GIN2 ELISA Kit, GLUCOSE INSENSITIVE 2 ELISA Kit, HEXOKINASE ELISA Kit, hexokinase 1 ELISA Kit, im:7148527 ELISA Kit, wu:fc09d08 ELISA Kit, wu:fc16e02 ELISA Kit, wu:fc21e02 ELISA Kit, wu:fq14b11 ELISA Kit, zgc:55790 ELISA Kit, zgc:77618 ELISA Kit, hexokinase-1 ELISA Kit, HK1 ELISA Kit, BB404130 ELISA Kit, Hk-1 ELISA Kit, Hk1-s ELISA Kit, dea ELISA Kit, mHk1-s ELISA Kit, HEXOKIN ELISA Kit, Hxk1 ELISA Kit, NtHxK1 ELISA Kit, NtHxk ELISA Kit, hexokinase ELISA Kit, StHK1 ELISA Kit, hxk ELISA Kit, hk1-A ELISA Kit, hexokinase 1 ELISA Kit, hexokinase-1 ELISA Kit, Hexokinase 1 ELISA Kit, hexokinase 1, putative ELISA Kit, hexokinase 1 L homeolog ELISA Kit, HK1 ELISA Kit, hk1 ELISA Kit, LOC100136279 ELISA Kit, Hex1 ELISA Kit, HXK1 ELISA Kit, Hk1 ELISA Kit, TA19800 ELISA Kit, hxk1 ELISA Kit, LOC107797523 ELISA Kit, LOC102604144 ELISA Kit, hk1.L ELISA Kit

Hintergrund:

Gene Name: Hexokinase 1

Gene Aliases: HK1-Ta, HK1-Tb, HK1-Tc, HKI, HXK1, Brain form hexokinase

Gen-ID: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effekt