HBEGF ELISA Kit
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- Target Alle HBEGF ELISA Kits anzeigen
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 15.6 pg/mL - 1000 pg/mL
- Untere Nachweisgrenze
- 15.6 pg/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and analogues was observed.
- Sensitivität
- 6.1 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle HBEGF ELISA Kits anzeigen
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
- Andere Bezeichnung
- Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF Produkte)
- Synonyme
- hbegf ELISA Kit, zgc:165443 ELISA Kit, HBEGF ELISA Kit, zgc:175091 ELISA Kit, DTR ELISA Kit, DTS ELISA Kit, DTSF ELISA Kit, HEGFL ELISA Kit, Dtr ELISA Kit, GFHB ELISA Kit, Hb-egf ELISA Kit, Hegfl ELISA Kit, AW047313 ELISA Kit, Dts ELISA Kit, HB-EGF ELISA Kit, heparin-binding EGF-like growth factor b ELISA Kit, heparin binding EGF like growth factor ELISA Kit, Heparin-binding EGF-like growth factor ELISA Kit, heparin-binding EGF-like growth factor a ELISA Kit, heparin-binding EGF-like growth factor ELISA Kit, hbegfb ELISA Kit, HBEGF ELISA Kit, hbegf ELISA Kit, hbegfa ELISA Kit, Hbegf ELISA Kit
- Hintergrund
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Gene Name: Heparin Binding Epidermal Growth Factor Like Growth Factor
Gene Aliases: HB-EGF, DTR, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor, Proheparin-binding EGF-like growth factor, Diphtheria toxin receptor
- Pathways
- RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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