Sonic Hedgehog ELISA Kit

Produktnummer ABIN5655006

Produktdetails Sonic Hedgehog ELISA Kit

Target: Sonic Hedgehog (SHH)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Hedgehog Homolog, Sonic (SHH). No significant cross-reactivity or interference between Hedgehog Homolog, Sonic (SHH) and analogues was observed.

Sensitivität: 30 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Sonic (SHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Homolog, Sonic (SHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Homolog, Sonic (SHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hedgehog Homolog, Sonic (SHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Hedgehog Homolog, Sonic (SHH Produkte)

Synonyme: HHG1 ELISA Kit, HLP3 ELISA Kit, HPE3 ELISA Kit, MCOPCB5 ELISA Kit, SMMCI ELISA Kit, TPT ELISA Kit, TPTPS ELISA Kit, 9530036O11Rik ELISA Kit, Dsh ELISA Kit, Hhg1 ELISA Kit, Hx ELISA Kit, Hxl3 ELISA Kit, M100081 ELISA Kit, fc83d08 ELISA Kit, shh ELISA Kit, syu ELISA Kit, vhh-1 ELISA Kit, vhh1 ELISA Kit, wu:fc83d08 ELISA Kit, Xhh ELISA Kit, hedgehog ELISA Kit, xshh ELISA Kit, SHH ELISA Kit, twh ELISA Kit, twhh ELISA Kit, sonic hedgehog ELISA Kit, sonic hedgehog a ELISA Kit, sonic hedgehog L homeolog ELISA Kit, sonic hedgehog protein A ELISA Kit, sonic hedgehog b ELISA Kit, SHH ELISA Kit, Shh ELISA Kit, shha ELISA Kit, shh.L ELISA Kit, shh ELISA Kit, shhb ELISA Kit

Hintergrund:

Gene Name: Hedgehog Homolog, Sonic

Gene Aliases: HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

Gen-ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development