Ghrelin CLIA Kit

Produktnummer ABIN5654548

Das Huhn Ghrelin ELISA Kit (ABIN5654548) ist ein Chemiluminescent ELISA Kit zur Detektion von Huhn Ghrelin.

Kurzübersicht für Ghrelin CLIA Kit (ABIN5654548)

Target: Ghrelin (GHRL)

Reaktivität: Huhn

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 39.06 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Produktdetails Ghrelin CLIA Kit

Untere Nachweisgrenze: 39.06 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.

Sensitivität: 17.31 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Ghrelin (GHRL). A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Ghrelin (GHRL) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Ghrelin (GHRL)

Andere Bezeichnung: Ghrelin

Hintergrund:

Gene Name: Ghrelin

Gene Aliases: MTLRP, Growth Hormone-Releasing Peptide

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour