GALT ELISA Kit

Produktnummer ABIN5654467

Produktdetails GALT ELISA Kit

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Galactose-1-Phosphate Uridylyltransferase (GALT). No significant cross-reactivity or interference between Galactose-1-Phosphate Uridylyltransferase (GALT) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactose-1-Phosphate Uridylyltransferase (GALT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactose-1-Phosphate Uridylyltransferase (GALT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactose-1-Phosphate Uridylyltransferase (GALT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galactose-1-Phosphate Uridylyltransferase (GALT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactose-1-Phosphate Uridylyltransferase (GALT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactose-1-Phosphate Uridylyltransferase (GALT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GALT

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Andere Bezeichnung: Galactose-1-Phosphate Uridylyltransferase (GALT Produkte)

Synonyme: GALT ELISA Kit, AW553376 ELISA Kit, CG9232 ELISA Kit, Dmel\\CG9232 ELISA Kit, dGALT ELISA Kit, galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridylyltransferase, GalT ELISA Kit, probable galactose-1-phosphate uridylyltransferase galtb [second part] ELISA Kit, UDP-glucose--galactose-1-phosphate uridylyltransferase ELISA Kit, GalT galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridyl transferase ELISA Kit, Galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridylyltransferase S homeolog ELISA Kit, GALT ELISA Kit, galT ELISA Kit, Galt ELISA Kit, CNM00620 ELISA Kit, Mrub_2813 ELISA Kit, Mesil_2395 ELISA Kit, Trad_0989 ELISA Kit, Igag_1846 ELISA Kit, VDIS_RS10425 ELISA Kit, Intca_2810 ELISA Kit, Marky_2157 ELISA Kit, Selsp_1876 ELISA Kit, Trebr_2368 ELISA Kit, Halhy_5538 ELISA Kit, Theth_1357 ELISA Kit, galt.S ELISA Kit

Hintergrund:

Gene Name: Galactose-1-Phosphate Uridylyltransferase

Gene Aliases: UDP-glucose--hexose-1-phosphate uridylyltransferase

Gen-ID: 298003

UniProt: P43424