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GALT ELISA Kit

GALT Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674828
  • Target Alle GALT ELISA Kits anzeigen
    GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
    Reaktivität
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of this index.
    Sensitivität
    0.134 ng/mL
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Testdurchführung

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handhabung
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Haltbarkeit
    12 months
  • Target Alle GALT ELISA Kits anzeigen
    GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
    Abstract
    GALT Produkte
    Synonyme
    GALT ELISA Kit, AW553376 ELISA Kit, CG9232 ELISA Kit, Dmel\\CG9232 ELISA Kit, dGALT ELISA Kit, galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridylyltransferase, GalT ELISA Kit, probable galactose-1-phosphate uridylyltransferase galtb [second part] ELISA Kit, UDP-glucose--galactose-1-phosphate uridylyltransferase ELISA Kit, GalT galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridyl transferase ELISA Kit, Galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridylyltransferase S homeolog ELISA Kit, GALT ELISA Kit, galT ELISA Kit, Galt ELISA Kit, CNM00620 ELISA Kit, Mrub_2813 ELISA Kit, Mesil_2395 ELISA Kit, Trad_0989 ELISA Kit, Igag_1846 ELISA Kit, VDIS_RS10425 ELISA Kit, Intca_2810 ELISA Kit, Marky_2157 ELISA Kit, Selsp_1876 ELISA Kit, Trebr_2368 ELISA Kit, Halhy_5538 ELISA Kit, Theth_1357 ELISA Kit, galt.S ELISA Kit
    Hintergrund
    Alternative name: UDP-glucose--hexose-1-phosphate uridylyltransferase
    Gen-ID
    2592
    UniProt
    P07902
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