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FGFR3 ELISA Kit

FGFR3 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5654308
  • Target Alle FGFR3 ELISA Kits anzeigen
    FGFR3 (Fibroblast Growth Factor Receptor 3 (FGFR3))
    Reaktivität
    • 5
    • 3
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor Receptor 3 (FGFR3). No significant cross-reactivity or interference between Fibroblast Growth Factor Receptor 3 (FGFR3) and analogues was observed.
    Sensitivität
    0.059 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Receptor 3 (FGFR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor Receptor 3 (FGFR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor Receptor 3 (FGFR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor Receptor 3 (FGFR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor 3 (FGFR3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor 3 (FGFR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle FGFR3 ELISA Kits anzeigen
    FGFR3 (Fibroblast Growth Factor Receptor 3 (FGFR3))
    Andere Bezeichnung
    Fibroblast Growth Factor Receptor 3 (FGFR3 Produkte)
    Synonyme
    FGFR3 ELISA Kit, LOC100034114 ELISA Kit, ACH ELISA Kit, CD333 ELISA Kit, CEK2 ELISA Kit, HSFGFR3EX ELISA Kit, JTK4 ELISA Kit, FR3 ELISA Kit, Fgfr-3 ELISA Kit, Flg-2 ELISA Kit, HBGFR ELISA Kit, Mfr3 ELISA Kit, sam3 ELISA Kit, fc27h01 ELISA Kit, wu:fc27h01 ELISA Kit, fibroblast growth factor receptor 3 ELISA Kit, fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) S homeolog ELISA Kit, FGFR3 ELISA Kit, fgfr3.S ELISA Kit, Fgfr3 ELISA Kit, fgfr3 ELISA Kit
    Hintergrund

    Gene Name: Fibroblast Growth Factor Receptor 3

    Gene Aliases: CD333, ACH, CEK2, JTK4, Achondroplasia, Thanatophoric Dwarfism

    Gen-ID
    2261
    UniProt
    P22607
    Pathways
    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Growth Factor Binding
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