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FGFR2 ELISA Kit

FGFR2 Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5654307
  • Target Alle FGFR2 ELISA Kits anzeigen
    FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
    Reaktivität
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.25 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.25 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor Receptor 2 (FGFR2). No significant cross-reactivity or interference between Fibroblast Growth Factor Receptor 2 (FGFR2) and analogues was observed.
    Sensitivität
    12.2 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Receptor 2 (FGFR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor Receptor 2 (FGFR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor Receptor 2 (FGFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor Receptor 2 (FGFR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor 2 (FGFR2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor 2 (FGFR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle FGFR2 ELISA Kits anzeigen
    FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
    Andere Bezeichnung
    Fibroblast Growth Factor Receptor 2 (FGFR2 Produkte)
    Synonyme
    FGFR2 ELISA Kit, FGFR2IIIb ELISA Kit, bek ELISA Kit, bfr-1 ELISA Kit, cd332 ELISA Kit, cek3 ELISA Kit, cfd1 ELISA Kit, ect1 ELISA Kit, fgfr-2 ELISA Kit, jws ELISA Kit, k-sam ELISA Kit, kgfr ELISA Kit, tk14 ELISA Kit, tk25 ELISA Kit, xfgfr2 ELISA Kit, DKFZp469H1521 ELISA Kit, BBDS ELISA Kit, BEK ELISA Kit, BFR-1 ELISA Kit, CD332 ELISA Kit, CEK3 ELISA Kit, CFD1 ELISA Kit, ECT1 ELISA Kit, JWS ELISA Kit, K-SAM ELISA Kit, KGFR ELISA Kit, TK14 ELISA Kit, TK25 ELISA Kit, AU043015 ELISA Kit, AW556123 ELISA Kit, Bek ELISA Kit, Fgfr-2 ELISA Kit, Fgfr-7 ELISA Kit, Fgfr7 ELISA Kit, KGFRTr ELISA Kit, svs ELISA Kit, FGF-R ELISA Kit, fgfr72IIIb ELISA Kit, FGFR ELISA Kit, fc56c05 ELISA Kit, wu:fc56c05 ELISA Kit, fibroblast growth factor receptor 2 ELISA Kit, fibroblast growth factor receptor 2 L homeolog ELISA Kit, FGFR2 ELISA Kit, LOC100084641 ELISA Kit, fgfr2 ELISA Kit, Fgfr2 ELISA Kit, fgfr2.L ELISA Kit
    Hintergrund

    Gene Name: Fibroblast Growth Factor Receptor 2

    Gene Aliases: CD332, BEK, BFR1, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, Bacteria-Expressed Kinase,Keratinocyte Growth Factor Receptor, Craniofacial Dysostosis 1, Crouzon Syndrome

    Gen-ID
    2263
    UniProt
    P21802
    Pathways
    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding
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