FGF7 ELISA Kit (Fibroblast Growth Factor 7)

Details for Product FGF7 ELISA Kit No. ABIN5654285, Anbieter: Anmelden zum Anzeigen
Antigen
  • HBGF-7
  • KGF
  • Kgf
  • kgf
  • FGF-7
  • fgf7
  • fibroblast growth factor 7
  • fibroblast growth factor 7 L homeolog
  • FGF7
  • Fgf7
  • fgf7
  • fgf7.L
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
24
17
13
10
4
3
3
3
2
1
1
1
1
Methodentyp
Competition ELISA
Detektionsbereich
12.35 pg/mL - 1000 pg/mL
Untere Nachweisgrenze
12.35 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Proben Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 7 (FGF7). No significant cross-reactivity or interference between Fibroblast Growth Factor 7 (FGF7) and analogues was observed.
Sensitivität 5.37 pg/mL
Andere Bezeichnung Fibroblast Growth Factor 7 (FGF7 ELISA Kit Abstract)
Hintergrund

Gene Name: Fibroblast Growth Factor 7

Gene Aliases: KGF, HBGF7, Keratinocyte Growth Factor, Heparin-binding growth factor 7

Pathways RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
Kommentare

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer 2 h
Plattentyp Pre-coated
Protokoll This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 7 (FGF7) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 7 (FGF7) and unlabeled Fibroblast Growth Factor 7 (FGF7) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 7 (FGF7). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 7 (FGF7) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 7 (FGF7) in the sample.
Testpräzision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 7 (FGF7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 7 (FGF7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Haltbarkeit 4-8 months
Haben Sie etwas anderes gesucht?