FGF1 CLIA Kit

Produktnummer ABIN5654173

Produktdetails FGF1 CLIA Kit

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reaktivität: Rind (Kuh)

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1). No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.

Sensitivität: 2.45 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 1, Acidic (FGF1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Andere Bezeichnung: Fibroblast Growth Factor 1, Acidic (FGF1 Produkte)

Synonyme: AFGF CLIA Kit, ECGF CLIA Kit, ECGF-beta CLIA Kit, ECGFA CLIA Kit, ECGFB CLIA Kit, FGF-1 CLIA Kit, FGF-alpha CLIA Kit, FGFA CLIA Kit, GLIO703 CLIA Kit, HBGF-1 CLIA Kit, HBGF1 CLIA Kit, Fgf1 CLIA Kit, FGF1 CLIA Kit, aFGF CLIA Kit, ecgf CLIA Kit, fgfa CLIA Kit, ecgfa CLIA Kit, ecgfb CLIA Kit, fgf-1 CLIA Kit, hbgf1 CLIA Kit, glio703 CLIA Kit, ecgf-beta CLIA Kit, fgf-alpha CLIA Kit, Dffrx CLIA Kit, Fam CLIA Kit, Fgf-1 CLIA Kit, Fgfa CLIA Kit, zgc:136885 CLIA Kit, LOC100221393 CLIA Kit, fgf1 CLIA Kit, zgc:73249 CLIA Kit, fibroblast growth factor 1 CLIA Kit, fibroblast growth factor 1 L homeolog CLIA Kit, fibroblast growth factor 1b CLIA Kit, fibroblast growth factor 1a CLIA Kit, FGF1 CLIA Kit, fgf1.L CLIA Kit, fgf1 CLIA Kit, Fgf1 CLIA Kit, fgf1b CLIA Kit, LOC100221393 CLIA Kit, fgf1a CLIA Kit

Hintergrund:

Gene Name: Fibroblast Growth Factor 1, Acidic

Gene Aliases: ECGF, AFGF, ECGFA, ECGFB, FGF-Alpha, FGFA, HBGF1, ECGFB, HBGF1, Acidic Fibroblast Growth Factor, heparin-binding growth factor 1, Endothelial Cell Growth Factor, Beta

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung