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DDR2 ELISA Kit

DDR2 Reaktivität: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5653696
  • Target Alle DDR2 ELISA Kits anzeigen
    DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
    Reaktivität
    • 7
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    1.56 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Discoidin Domain Receptor Family, Member 2 (DDR2). No significant cross-reactivity or interference between Discoidin Domain Receptor Family, Member 2 (DDR2) and analogues was observed.
    Sensitivität
    0.63 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discoidin Domain Receptor Family, Member 2 (DDR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discoidin Domain Receptor Family, Member 2 (DDR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discoidin Domain Receptor Family, Member 2 (DDR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Discoidin Domain Receptor Family, Member 2 (DDR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Discoidin Domain Receptor Family, Member 2 (DDR2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Discoidin Domain Receptor Family, Member 2 (DDR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle DDR2 ELISA Kits anzeigen
    DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
    Andere Bezeichnung
    Discoidin Domain Receptor Family, Member 2 (DDR2 Produkte)
    Synonyme
    MIG20a ELISA Kit, NTRKR3 ELISA Kit, TKT ELISA Kit, TYRO10 ELISA Kit, AW495251 ELISA Kit, Ntrkr3 ELISA Kit, tyro10 ELISA Kit, DDR2 ELISA Kit, Tyro10 ELISA Kit, ddr2 ELISA Kit, si:ch211-193c2.1 ELISA Kit, discoidin domain receptor tyrosine kinase 2 ELISA Kit, discoidin domain receptor family, member 2 ELISA Kit, discoidin domain receptor family, member 2, like ELISA Kit, DDR2 ELISA Kit, Ddr2 ELISA Kit, ddr2l ELISA Kit
    Hintergrund

    Gene Name: Discoidin Domain Receptor Family, Member 2

    Gene Aliases: CD167b, MIG20a, NTRKR3, TKT, TYRO10, Discoidin Domain Receptor Tyrosine Kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT

    Gen-ID
    4921
    UniProt
    Q16832
    Pathways
    RTK Signalweg
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