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Cytochrome C ELISA Kit

CYCS Reaktivität: Ratte, Maus Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5653488
  • Target Alle Cytochrome C (CYCS) ELISA Kits anzeigen
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Reaktivität
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    1.56 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cytochrome C, Somatic (CYCS). No significant cross-reactivity or interference between Cytochrome C, Somatic (CYCS) and analogues was observed.
    Sensitivität
    0.59 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C, Somatic (CYCS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C, Somatic (CYCS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C, Somatic (CYCS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C, Somatic (CYCS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C, Somatic (CYCS) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C, Somatic (CYCS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Cytochrome C (CYCS) ELISA Kits anzeigen
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Andere Bezeichnung
    Cytochrome C, Somatic (CYCS Produkte)
    Synonyme
    CYC ELISA Kit, HCS ELISA Kit, THC4 ELISA Kit, cyct ELISA Kit, cyc ELISA Kit, hcs ELISA Kit, CYCS ELISA Kit, cytc ELISA Kit, CYCSA ELISA Kit, CG17903 ELISA Kit, Cyt C ELISA Kit, Cyt c ELISA Kit, Cyt-c2 ELISA Kit, CytC-2 ELISA Kit, Cytc ELISA Kit, Cytc-p ELISA Kit, DC4 ELISA Kit, DMc01 ELISA Kit, Dmel\\CG17903 ELISA Kit, cyt c ELISA Kit, cyt c-p ELISA Kit, cyt-c-p ELISA Kit, cyt-c2 ELISA Kit, cyt.c ELISA Kit, cytochrome c ELISA Kit, dc4 ELISA Kit, pDMc01 ELISA Kit, wu:fk52b01 ELISA Kit, zgc:86706 ELISA Kit, ENSMUSG00000058927 ELISA Kit, cytochrome c, somatic ELISA Kit, cytochrome c, somatic S homeolog ELISA Kit, Cytochrome c proximal ELISA Kit, cytochrome c, somatic b ELISA Kit, cytochrome c, somatic pseudogene ELISA Kit, cytochrome c-like ELISA Kit, cytochrome c ELISA Kit, CYCS ELISA Kit, cycs.S ELISA Kit, cycs ELISA Kit, Cycs ELISA Kit, Cyt-c-p ELISA Kit, cycsb ELISA Kit, Gm10053 ELISA Kit, LOC100724240 ELISA Kit, CYC ELISA Kit, LOC100847700 ELISA Kit, LOC101107954 ELISA Kit, LOC106829744 ELISA Kit, LOC106992390 ELISA Kit, LOC102085712 ELISA Kit
    Hintergrund

    Gene Name: Cytochrome C, Somatic

    Gene Aliases: Cyt-C, HCS, CYC, CytC

    Pathways
    Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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